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Copyright (c) 2006 Department of Physiology, Tokyo Medical University. All rights reserved.

研究分野:
培養下で形成されるシナプスを用いた神経伝達物質放出のメカニズム、および伝達物質受容の研究
研究課題:
神経伝達物質放出、シナプス小胞サイクルを制御するシナプス前終末蛋白質、および伝達物質受容に関与するシナプス後細胞蛋白質の機能解析
主な方法:
リコンビナント蛋白、部分ペプチド、遺伝子を培養ラット上頸交感神経節細胞シナプスに導入して、シナプス伝達の変化を電気生理学的、およびイメージング手法を用いて解析
日本生理学会(常任幹事)、日本神経科学学会(専門会員)、北米神経科学学会会員、IBRO(International Brain Research Organization)
I have been studying on molecular mechanism of synaptic transmission employing electrophysiological measurements of responses recorded from a cholinergic model synapse formed between sympathetic ganglion neurons in long-term culture. Roles of proteins for neurotransmitter release have been extensively examined by perturbing their functions with injection of neurotoxins, synthetic peptides, recombinant proteins or with transfection of cDNA/siRNA (see update article, Ma and Mochida, Neurosci Res., 2007). With this approach, I have been demonstrated unique functions of N- and P/Q-type calcium channels (Neuron 1996, PNAS 1998, 2003a,b, Neuron 2008, J. Physiol. 2011, PNAS 2012, JBC 2013), SNARE-associated proteins (Syntaxin: Neuroscience 1995, J. Neurosci. 2002; Snapin: nature neurosci. 1999; Syntaphilin: Neuron 2000; SNAP-29: PNAS 2001, Tomosyn: JCB 2005, 2008; JBC 2009, 2010; VAMP2: Neuron 2010), synaptic vesicle associated proteins (Synaptotagmin: Neuroscience 1997; Doc2: PNAS 1998; SV2s: Mol. Cell. Neurosci. 2005, Mol Pharmacol. 2012; TRPM7: Neuron 2006; V-ATPase: Neuron 2010), G proteins (Goβγ J Physiol. 2005), active zone proteins (CAST: JCB 2004; SAD: Neuron 2006), motor proteins (Myosin II and V: Neuron 1994; J Physiol. 2005) in regulation of transmitter secretion, synaptic vesicle docking/priming and synaptic vesicle trafficking. Endocytic proteins (dynamin/amphyphysin, JBC 2009; dynamin 1, 2, 3 JBC 2013) and axonal mitochondria transport (KIF5/syntabulin, J Neurosci. 2009) are required to maintain synaptic transmission. The presynaptic (PNAS 2010) and postsynaptic metabotropic glutamate receptors functions have also been studied (J. Neurochem. 2003).
教材ダウンロード:
終了しました【2014.01.06】

電話:03-3351-6141内線248/FAX:03-5379-0658

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